Journal: STAR Protocols

Article Title: Protocol for whole-cell patch-clamp recording and post hoc identification of hippocampal CA2 pyramidal neurons in adult mouse brain slices

doi: 10.1016/j.xpro.2026.104470

Figure Lengend Snippet: Pyramidal neuron in the hippocampal CA2 subfield (A) Confocal light-micrograph of a biocytin-filled pyramidal neuron stained with Alexa 564 conjugated streptavidin. (B) Labeling of RGS14 immunoreactivity using Alexa 647. Dashed white lines indicate approximative proximal-distal boundaries of CA2. (C) Overlay of biocytin and RGS14 labeling. Note the presence of recorded pyramidal neuron in the CA2 stained subfield.

Article Snippet: Streptavidin, Alexa FluorTM 594 Conjugate , Fisher Scientific , Cat# S11227.

Techniques: Staining, Labeling

Journal: STAR Protocols

Article Title: Protocol for whole-cell patch-clamp recording and post hoc identification of hippocampal CA2 pyramidal neurons in adult mouse brain slices

doi: 10.1016/j.xpro.2026.104470

Figure Lengend Snippet: Identification of the hippocampal CA2 with specific regional markers (A) Hippocampal cellular organization (DAPI, top in blue), expression of the putative CA2 markers WFA (second from the top in red) and PCP4 (third from the top in light grey) and overlay (bottom). Dotted yellow square is for enlarged views. WFA staining is revealed using Alexa 555 via biotin–streptavidin system conjugate and PCP4 staining using Alexa 647 via secondary antibody. Images are captured with a 63X oil objective mounted on a Zeiss LSM880 in Airyscan mode. (B) Expanded view of the images in (A). The yellow dotted line represent the CA1/CA2 boundary. Note the clear transition in the distribution of the DAPI staining that coincides with the WFA and PCP4 staining and colocalization of WFA and PCP4 markers in CA2.

Article Snippet: Streptavidin, Alexa FluorTM 594 Conjugate , Fisher Scientific , Cat# S11227.

Techniques: Expressing, Staining